Electron microscopic method for determining the concentration of influenza virus antihemagglutinin antibodies of the IgG class
Identifieur interne : 002303 ( Main/Exploration ); précédent : 002302; suivant : 002304Electron microscopic method for determining the concentration of influenza virus antihemagglutinin antibodies of the IgG class
Auteurs : R. Stachan [Allemagne] ; J. Drescher [Allemagne]Source :
- Journal of Virological Methods [ 0166-0934 ] ; 1987.
English descriptors
- Teeft :
- Acta pathol, Additional method, Antibody, Antibody concentration, Antibody concentrations, Antibody dilution, Antibody molecule, Antibody molecules, Antibody preparation, Antibody preparations, Antigenic, Antigenic determinants, Aqueous uranyl acetate solution, Binding avidity, Colloidal gold, Corresponding concentration, Corresponding values, Dilution, Drescher, Electron microscope, Electron microscopically, Electron microscopy, Gold particle, Gold particles, Influenza, Influenza virus, Influenza virus antibody, Influenza virus hemagglutinin, Molecule, Monoclonal, Monoclonal antibodies, Particle counts, Pattern test, Protein content, Room temperature, Screen magnification, Test sample, Twofold dilutions, Unbound, Unbound antibody, Unbound antibody molecules, Viral, Viral antigens, Virus, Virus particle, Virus particles, Virus surface.
Abstract
Abstract: A novel technique for the determination of the concentration of influenza virus antihemagglutinin antibody of the IgG class is described which is based on the following principle: graded doses of purified antibody are allowed to react with homologous virus, resulting in binding of a portion of antibody. The virus-bound antibody is electron microscopically counted after labeling with protein A-gold. Since Freundlich's λ-formula is valid for describing the relationship between antibody bound per virus particle and the corresponding concentration of unbound antibody, the values of bound antibody recorded can be used for calculating the concentration of unbound antibody. The antibody concentration of the test sample is obtained as sum of bound and unbound antibody. The antibody concentrations measured by use of this technique agreed well with chemically determined values.
Url:
DOI: 10.1016/0166-0934(87)90123-6
Affiliations:
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Stachan</name><affiliation wicri:level="3"><country xml:lang="fr" wicri:curation="lc">Allemagne</country><wicri:regionArea>Institute of Virology, Medical School of Hannover, Hannover</wicri:regionArea><placeName><region type="land" nuts="2">Basse-Saxe</region><settlement type="city">Hanovre</settlement></placeName></affiliation></author><author><name sortKey="Drescher, J" sort="Drescher, J" uniqKey="Drescher J" first="J." last="Drescher">J. 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The virus-bound antibody is electron microscopically counted after labeling with protein A-gold. Since Freundlich's λ-formula is valid for describing the relationship between antibody bound per virus particle and the corresponding concentration of unbound antibody, the values of bound antibody recorded can be used for calculating the concentration of unbound antibody. The antibody concentration of the test sample is obtained as sum of bound and unbound antibody. The antibody concentrations measured by use of this technique agreed well with chemically determined values.</div></front></TEI><affiliations><list><country><li>Allemagne</li></country><region><li>Basse-Saxe</li></region><settlement><li>Hanovre</li></settlement></list><tree><country name="Allemagne"><region name="Basse-Saxe"><name sortKey="Stachan, R" sort="Stachan, R" uniqKey="Stachan R" first="R." last="Stachan">R. Stachan</name></region><name sortKey="Drescher, J" sort="Drescher, J" uniqKey="Drescher J" first="J." last="Drescher">J. Drescher</name></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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